Diloxanide furoate (1.95-2.5 μg/ml) has potent activity against different strains of E. histolytica , it is against BYso (grown with bacteria), SFL3 (grown with Chrithidia sp.), and SFL3 (grown axenically) with MIC values of 2.5 μg/ml, 2.5 μg/ml and 1.95 μg/ml, respectively.

Diloxanide furoate (oral administraion; 75-200 mg/kg; 3 days; once daily) is effective at different dose of dayin weanling rats. At 200 mg/kg, 100% of the treated rats are cured and no amoebic lesions are observed in the caecum. Besides, 85%,77%, and 44.4% of the treated rats are cured at the dose 150 mg/kg, 100 mg/kg, and 75 mg/kg, respectively. The ED ₅₀ value is 77.9 mg/kg for this agent in rats.